期刊
JOURNAL OF NEUROTRAUMA
卷 38, 期 12, 页码 1702-1716出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/neu.2020.7309
关键词
confocal; glutamate; inflammation; mesenchymal; Muller; neurodegeneration
资金
- Department of Defense [W81XWH-16-1-0076, W81XWH-16-1-0761]
- National Eye Institute [EY023427]
- Research to Prevent Blindness
- Neuroscience Institute, UTHSC
The study suggests a potential neuroprotective role of ASC-CCM in rescuing visual deficits and pathologies of mild traumatic brain injury by restoring the health of Muller cells.
Concentrated conditioned media from adipose tissue-derived mesenchymal stem cells (ASC-CCM) show promise for retinal degenerative diseases. In this study, we hypothesized that ASC-CCM could rescue retinal damage and thereby improve visual function by acting through Muller glia in mild traumatic brain injury (mTBI). Adult C57Bl/6 mice were subjected to a 50-psi air pulse on the left side of the head, resulting in an mTBI. After blast injury, 1 mu L (similar to 100 ng total protein) of human ASC-CCM was delivered intravitreally and followed up after 4 weeks for visual function assessed by electroretinogram and histopathological markers for Muller cell-related markers. Blast mice that received ASC-CCM, compared with blast mice that received saline, demonstrated a significant improvement in a- and b-wave response correlated with a 1.3-fold decrease in extracellular glutamate levels and a concomitant increase in glutamine synthetase (GS), as well as the glutamate transporter (GLAST) in Muller cells. Additionally, an increase in aquaporin-4 (AQP4) in Muller cells in blast mice received saline restored to normal levels in blast mice that received ASC-CCM. In vitro studies on rMC-1 Muller glia exposed to 100 ng/mL glutamate or RNA interference knockdown of GLAST expression mimicked the increased Muller cell glial fibrillary acidic protein (a marker of gliosis) seen with mTBI, and suggested that an increase in glutamate and/or a decrease in GLAST might contribute to the Muller cell activation in vivo. Taken together, our data suggest a novel neuroprotective role for ASC-CCM in the rescue of the visual deficits and pathologies of mTBI via restoration of Muller cell health.
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