期刊
JOURNAL OF NEUROGENETICS
卷 35, 期 1, 页码 33-44出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/01677063.2020.1853722
关键词
Drosophila; gut; GAL4; regional; cell-type specificity; resource
资金
- National Research Foundation of Korea (NRF) [NRF-2017M3A9B8069650]
- Samsung Science and Technology Foundation [SSTF-BA-1802-11]
- GIST Research Institute (GRI) grant - GIST in 2020
- National Creative Research Initiative Program [2015R1A3A2033475]
- NRF [NRF-2020R1A2C2009865, NRF-2020R1F1A1070665, NRF-2017R1A2B4007280, NRF-2018R1D1A1B07049280, NRF-2019R1I1A1A01061499, NRF-2019R1I1A1A01059606, NRF-2020R1I1A1A01072255]
- National Research Foundation of Korea [2017M3A9B8069650, 2015R1A3A2033475] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This study provides a valuable resource of GAL4 lines and split-GAL4 lines to target and manipulate different cell types and regions in the Drosophila gut, facilitating a more precise investigation of gut cells that regulate important biological processes.
The gastrointestinal tract in the adult Drosophila serves as a model system for exploring the mechanisms underlying digestion, absorption and excretion, stem cell plasticity, and inter-organ communication, particularly through the gut-brain axis. It is also useful for studying the cellular and adaptive responses to dietary changes, alterations in microbiota and immunity, and systematic and endocrine signals. Despite the various cell types and distinct regions in the gastrointestinal tract, few tools are available to target and manipulate the activity of each cell type and region, and their gene expression. Here, we report 353 GAL4 lines and several split-GAL4 lines that are expressed in enteric neurons (ENs), progenitors (ISCs and EBs), enterocytes (ECs), enteroendocrine cells (EEs), or/and other cell types that are yet to be identified in distinct regions of the gut. We had initially collected approximately 600 GAL4 lines that may be expressed in the gut based on RNA sequencing data, and then crossed them to UAS-GFP to perform immunohistochemistry to identify those that are expressed selectively in the gut. The cell types and regional expression patterns that are associated with the entire set of GAL4 drivers and split-GAL4 combinations are annotated online at http://kdrc.kr/index.php (K-Gut Project). This GAL4 resource can be used to target specific populations of distinct cell types in the fly gut, and therefore, should permit a more precise investigation of gut cells that regulate important biological processes.
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