4.4 Article

Acupuncture Ameliorates Neuronal Cell Death, Inflammation, and Ferroptosis and Downregulated miR-23a-3p After Intracerebral Hemorrhage in Rats

期刊

JOURNAL OF MOLECULAR NEUROSCIENCE
卷 71, 期 9, 页码 1863-1875

出版社

SPRINGERNATURE
DOI: 10.1007/s12031-020-01770-x

关键词

Acupuncture; Ferroptosis; Intracerebral hemorrhage; MicroRNA-23a-3p; Baihui (DU20); Qubin (GB7)

资金

  1. National Natural Science Foundation of China [81904305]
  2. Heilongjiang University of Chinese Medicine Doctoral Innovation Foundation [2017bs05]
  3. Heilongjiang Provincial Postdoctoral Science Foundation [LBH-Z12249]
  4. Project of Science and Technology of Education Department of Heilongjiang Province [12531633]
  5. Foundation of Heilongjiang University of Chinese Medicine [2019PT10]

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Baihui-penetrating-Qubin acupuncture may alleviate neuronal cell death, inflammation, and ferroptosis after intracerebral hemorrhage by down-regulating miR-23a-3p.
Baihui-penetrating-Qubin acupuncture is frequently used to treat intracerebral hemorrhage (ICH) in China. Acupuncture affects multiple microRNAs in diseases. MicroRNA-23a-3p (miR-23a-3p) has been demonstrated to be up-regulated in ICH patients. Herein, the effect of Baihui-penetrating-Qubin acupuncture on miR-23a-3p expression after ICH and the role of miR-23a-3p in ICH were discussed. A rat model of ICH was induced by infusing autologous blood into caudate nucleus. Acupuncture was performed after ICH once a day for 30 min. After 3 consecutive days of acupuncture, the neurobehavioral function, brain edema, neuronal cell death, inflammation, ferroptosis, nuclear factor E2-like 2 (NFE2L2) signaling and miR-23a-3p levels in brain tissues were analyzed. Additionally, antagomiR-23a-3p was injected into rats 3 days prior to ICH modeling to analyze the function of miR-23a-3p in neuronal cell death, inflammation, ferroptosis, and NFE2L2 signaling. Acupuncture relieved the ICH-induced neurological function deficits, increases in brain water content and Fluoro-Jade B (FJB)-positive cells and release of proinflammatory cytokines. Acupuncture also alleviated ferroptosis and decreased miR-23a-3p expression, as evidenced by the increased NFE2L2 nuclear translocation and expressions of heme oxygenase-1 and glutathione peroxidase 4 and the decreased iron and malondialdehyde contents and reactive oxygen species accumulation. Additionally, antagomiR-23a-3p inhibited the ICH-induced increases in FJB-positive cells, release of proinflammatory cytokines, ferroptosis, and promoted NFE2L2 activation. Notably, the binding site of miR-23a-3p existed in NFE2L2. Taken together, acupuncture may alleviate the neuronal cell death, inflammation, and ferroptosis after ICH by down-regulating miR-23a-3p. This study provides a potential mechanism underlying the Baihui-penetrating-Qubin acupuncture improving the early injury after ICH.

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