期刊
JOURNAL OF MEDICAL VIROLOGY
卷 93, 期 7, 页码 4616-4619出版社
WILEY
DOI: 10.1002/jmv.26791
关键词
mutation; nsp7; nsp8; RdRp; SARS‐ CoV‐ 2
类别
Specific mutations in nsp7 and nsp8 proteins identified in viral genomes may play a role in stabilizing the replication/transcription complex by affecting the formation of the RdRp-nsp7-nsp8 supercomplex.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA-dependent RNA polymerase (RdRp) has been identified to be a mutation hot spot, with the P323L mutation being commonly observed in viral genomes isolated from North America. RdRp forms a complex with nonstructural proteins nsp7 and nsp8 to form the minimal replication/transcription machinery required for genome replication. As mutations in RdRp may affect formation of the RdRp-nsp7-nsp8 supercomplex, we analyzed viral genomes to identify mutations in nsp7 and nsp8 protein sequences. Based on in silico analysis of predicted structures of the supercomplex comprising of native and mutated proteins, we demonstrate that specific mutations in nsp7 and nsp8 proteins may have a role in stabilization of the replication/transcription complex.
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