期刊
JOURNAL OF MEDICAL ENTOMOLOGY
卷 58, 期 4, 页码 1525-1535出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/jme/tjaa269
关键词
next-generation sequencing; microbiome; pathogen discovery
资金
- Steven & Alexandra Cohen Foundation
- Global Lyme Alliance
Over the past 30 years, molecular assays have largely replaced classical methods for detection of tick-borne agents, leading to a rapid increase in newly characterized tick-borne agents. High throughput sequencing has enabled prompt identification of new pathogens, but little is currently known about the majority of these agents.
Within the past 30 yr molecular assays have largely supplanted classical methods for detection of tick-borne agents. Enhancements provided by molecular assays, including speed, throughput, sensitivity, and specificity, have resulted in a rapid increase in the number of newly characterized tick-borne agents. The use of unbiased high throughput sequencing has enabled the prompt identification of new pathogens and the examination of tick microbiomes. These efforts have led to the identification of hundreds of new tick-borne agents in the last decade alone. However, little is currently known about the majority of these agents beyond their phylogenetic classification. Our article outlines the primary methods involved in tick-borne agent discovery and the current status of our understanding of tick-borne agent diversity.
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