期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 178, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2020.107519
关键词
Chromogenic in situ hybridization; Quantitative PCR; Gamma proteobacteria; Siliqua patula; Razor Clam; Nuclear Inclusion X; NIX
类别
资金
- NOAA Hollings Scholar Program
The study found that nuclear inclusion X (NIX) infection in Pacific razor clams is associated with mass mortality in Washington coastal beaches. A detection method was developed and its specificity was confirmed. The research indicated significant differences in infection rates between razor clams from two different locations.
Nuclear inclusion X (NIX) is a gamma proteobacteria that infects the nuclei of gill epithelial cells in Pacific razor clams. NIX has been associated with clam die-offs in coastal Washington. A quantitative PCR (qPCR) assay was developed to detect NIX in Pacific razor clams, and assay specificity was confirmed by chromogenic in situ hybridization (CISH). Both tests were applied to evaluate NIX infections in wild Pacific razor clams collected during spring 2019. Consistent with results from earlier histopathological assessments, qPCR and CISH indicated 100% prevalence in razor clams from two Washington beaches and 0% prevalence from two Alaskan beaches.
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