期刊
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
卷 95, 期 -, 页码 148-155出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jiec.2020.12.015
关键词
Human adipose tissue; Alginate-fat scaffold; Adipose tissue regeneration
资金
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT, and Future Planning and the Korea government (MSIT) [2020M3A914039045, 2016M3A9B4919711, 2018M3A9E9071162]
- National Research Foundation of Korea [2018M3A9E9071162] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The study utilized human adipose tissue and alginate to fabricate a scaffold material that showed enhanced adipogenesis ability through non-cytotoxic cross-linking. Alginate protected adipose tissue, leading to better survival and shape preservation in in vivo experiments.
Autologous fat and synthetic and natural polymers have been used as materials and scaffolds in adipose tissue (AD) regeneration, combining various polymers and biological components, including extracellular matrix molecules, decellularized matrix, and adipose-derived stem cells, which have been studied to improve the adipogenesis ability of scaffolds. This study used human AD as the scaffold material, with a great source of biomaterials closest to the human body. We report a simple and facile scaffold fabrication method using a natural polymer, alginate, and human AD and confirmed the adipogenesis ability in vivo. Scaffold fabrication was performed by simply mixing human AD with alginate and cross-linking in a non-cytotoxic way, using CaCl2 solution. This method allows facile control of the shape and mechanical properties of the scaffold, which has great advantages in medical and aesthetic applications. In vitro experiments demonstrated the viability and secretion of adipokines in the scaffold. Four weeks of in vivo experiments showed that alginate protected human AD, which aided in AD survival, and that volume and shape were better preserved. Furthermore, we confirmed that our scaffold enhanced the adipose regeneration of the host animal and increased adipogenesis of the transplanted human AD by gene expression analysis. (c) 2020 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
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