Long-term enrichment of anaerobic propionate-oxidizing consortia: Syntrophic culture development and growth optimization

Propionate is a quantitatively important methanogenic intermediate in anaerobic digesters and only limited number of microbes can utilize it under syntrophic association with methanogens. The syntrophic propionate oxidizing bacterias are known to be slow growers due to the low energy yield. Thus, propionate get accumulated frequently in anaerobic digesters and it negatively affect digester performance. In this study, propionate degrading consortia from four different seeding sources were enriched in sequential bath mode in two phases; first adaption phase with 1 g/L of propionate concentration and later, high-strength phase with 3 g/L. From 16s rRNA gene based metagenomics analysis of the former phase, four syntrophic microbial groups, Syntrophaceae, Syntrophomonadaceae, Methanobacterium and Methanosaeta were found to be dominant with complete degradation of propionate. The substrate accelerated microbial shifts were observed at high-strength phase with significant decrease of Syntrophaceae up to 26.9 %. Using Response Surface Methodology, pH 6.8-6.9 and temperature 34.5-34.9 C-omicron were found to be optimum growth conditions for the propionate degradation culture. Observed results could be useful to improve degradation efficiencies and obtained enriched culture can be used to recover propionate-accumulated digesters by bio-augmentation.

Automated summary

The study enriched propionate degrading consortia from four different seeding sources and identified dominant syntrophic microbial groups through 16s rRNA gene based metagenomics analysis. Accelerated microbial shifts were observed at high-strength phase with a significant decrease of Syntrophaceae up to 26.9%. Response Surface Methodology revealed that pH 6.8-6.9 and temperature 34.5-34.9 C-omicron were optimum growth conditions for the propionate degradation culture.