Alleviation of tributyltin-induced toxicity by diet and microplastics in the marine rotifer Brachionus koreanus

To determine the effects of tributyltin (TBT) upon multiple exposures of diet and microplastic in rotifer, in vivo life parameters were measured. In 10 mu g/L TBT-exposed rotifer, the 1 and 0.5 x diet groups resulted in repro-duction reduction. However, 10 x diet treatment showed no significant changes in the total fecundity, despite a decrease in daily reproduction. Besides, differences in the lifespan were observed in response to different diet regimens. TBT and/or MP-exposed parental rotifer (F0) showed a significant delay in the pre-reproductive day under 0.5 x diet regimen. In all dietary regimens, exposure to TBT and MP induced an increase in reactive oxygen species, but antioxidant activities were perturbed. To further verify the carryover effect of TBT toxicity, progeny rotifer (F1) obtained from 24 h TBT and/or MP-exposed F0 was used. Interestingly, the faster hatching rate was observed only in F1 obtained from 1 x diet regimen-exposed F0. However, in the 0.5 x diet, the total fecundity was reduced and the pattern of the daily reproduction was collapsed. Thus, the toxicity of TBT can be alleviated by MP and nutrition status, but TBT-induced toxicity and its carryover effect are inevitable.

Automated summary

The effects of TBT and microplastic exposure on rotifers were investigated, showing varied impacts on reproduction and lifespan under different dietary regimens. Reactive oxygen species increased and antioxidant activities were disrupted in response to TBT and/or microplastic exposure. The carryover effect of TBT toxicity was observed in progeny rotifers from exposed parental rotifers.