A smartphone-integrated multicolor fluorescence probe of bacterial spore biomarker: The combination of natural clay material and metal-organic frameworks

The metal-organic frameworks (MOFs) functionalized palygorskite (Pal) hybrid as a novel multicolor fluorescence probe for the detection of bacterial spore biomarker-dipicolinic acid (DPA), had been prepared via in-situ growth. The MOFs can effectively encapsulate dye molecules on the surface of Pal, and the rich carboxyl groups on its surface can coordinate with europium ions (Eu3+), forming a highly sensitive recognition group. The results indicated that the limit of detection (LOD) of this multicolor fluorescence probe was as low as 9.3 nM and was obviously lower than the amount of anthrax spores infecting the human body (60 mu M). Moreover, a wide linear range from 0 to 35 mu M was obtained. The high specific surface area of Pal, as well as the permanent porosity and suitable binding sites of Eu3+-doped MOFs may play a major role in the sensitivity and linear detection range. The multicolor fluorescence strategy made full use of the diversity of fluorescence signals collected by dye molecules and lanthanide ions, which can realize the real-time and on-site detection through the smartphone with a color-scanning application (APP). The practicability of this probe was further verified by detecting DPA released by non-infectious Bacillus subtilis.

Automated summary

A novel multicolor fluorescence probe for detecting bacterial spore biomarker-dipicolinic acid (DPA) was prepared using metal-organic frameworks (MOFs) functionalized palygorskite (Pal) hybrid. The probe exhibited high sensitivity with a low limit of detection and a wide linear range, and could be used for real-time detection through a smartphone application. The practicality and efficiency of the probe were verified through experiments.