期刊
JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
卷 336, 期 4, 页码 352-363出版社
WILEY
DOI: 10.1002/jez.b.23025
关键词
body color; crustacyanin gene; gene cloning; lipocalin gene family; RNA interference
资金
- National Key R&D Program of China [2018YFD0901302]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Natural Science Foundation of the Jiangsu Higher Education Institutions of China [17KJA240001]
- Open-end Funds of Jiangsu Key Laboratory of Marine Bioresources and Environment [SH20191203]
The study revealed the important role of the crustacyanin gene in body color variation and heavy metal stress resistance of Exopalaemon carinicauda, with RNA interference experiments confirming the expression patterns of different subtypes in different tissues. This sheds light on the mechanisms underlying body color variation in E. carinicauda.
Crustacyanin has the function of binding astaxanthin which is the best antioxidant, and plays an important role in the body color variation of crustaceans. To investigate the causes of body color variation of the ridgetail white prawn, Exopalaemon carinicauda, the present study obtained four subtypes of crustacyanin gene: C1, C2, A1, and A2. Based on fluorescence quantitative polymerase chain reaction, lipocalin-C1 is mainly expressed in the eyestalk, lipocalin-C2 is in the ventral nerve cord, and lipocalin-A1 and lipocalin-A2 are in subcutaneous adipose tissues. Under the inhibiting effect of Cd2+ stress, the expression of four subtypes first increases and then decreases within 24 h, and reaches the maximum at 6 or 12 h. RNA interference experiments showed a decrease in the expression of lipocalin genes in subcutaneous adipose tissue for each subtype, with the body color changing from transparent to red, and the dark red spots on the epidermis changing to bright red. Moreover, the blue protein in the subcutaneous adipose tissue largely disappeared, based on the light micrographs. In view of these findings, the crustacyanin gene appears to fulfill some function in the resistance to heavy metal stress and body color formation of E. carinicauda.
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