期刊
JOURNAL OF EXPERIMENTAL MEDICINE
卷 218, 期 3, 页码 -出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20200650
关键词
-
资金
- National Institutes of Health [AI125741, DK127526, AI153537, DK108557, DK107541, DK121747, AI137248]
- American Cancer Society Research Scholar Grant
- Advancing a Healthier Wisconsin Endowment Grant
- Cancer Research Institute Irvington Fellowship
- National Institute of General Medical Sciences [T32-GM080202]
Single-cell sequencing technology was used to track individual virus-specific CD4 T cells during an acute lymphocytic choriomeningitis virus (LCMV) infection, revealing previously unappreciated clonal diversity and cellular heterogeneity among virus-specific helper T cells. Despite most naive CD4 T cells giving rise to multiple lineages, approximately 28% of naive cells exhibited a preference towards either Th1 or T-FH cells, with the TCR structure influencing lineage decisions.
Tracking how individual naive T cells from a natural TCR repertoire clonally expand, differentiate, and make lineage choices in response to an infection has not previously been possible. Here, using single-cell sequencing technology to identify clones by their unique TCR sequences, we were able to trace the clonal expansion, differentiation trajectory, and lineage commitment of individual virus-specific CD4 T cells during an acute lymphocytic choriomeningitis virus (LCMV) infection. Notably, we found previously unappreciated clonal diversity and cellular heterogeneity among virus-specific helper T cells. Interestingly, although most naive CD4 T cells gave rise to multiple lineages at the clonal level, similar to 28% of naive cells exhibited a preferred lineage choice toward either Th1 or T-FH cells. Mechanistically, we found that TCR structure, in particular the CDR3 motif of the TCR alpha chain, skewed lineage decisions toward the T-FH cell fate.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据