期刊
JOURNAL OF DAIRY SCIENCE
卷 104, 期 3, 页码 2511-2519出版社
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2020-19282
关键词
chymosin; cheesemaking; electrochemical assay
资金
- National Natural Science Foundation of China (Beijing, China) [21764005, 21565029]
- Key Projects of Yunnan Natural Science Foundation (Kunming, China) [2018FA005]
- Key Research and Development Projects of Yunnan (Kunming, China) [2018BC005]
- Key Scientific and Technology Project of Yunnan (Kunming, China) [202002AE320005]
- Program for Excellent Young Talents of Yunnan University
- Program for Donglu Scholars of Yunnan University (Kunming, China)
A label-free electrochemical method was developed to determine chymosin activity in cheese production, with a synthesized peptide substrate assembled on an Au-deposited carbon electrode. The method showed high selectivity, stability, and a low detection limit. This fast and effective assay has potential applications in the quantification of chymosin activity in commercial rennet.
Chymosin is a predominant enzyme in rennet and is used in cheese production because of its excellent milk-clotting activity. Herein, we proposed a facile and label-free electrochemical method for determining chymosin activity based on a peptide-based enzyme substrate. The synthesized substrate peptide for chymosin was assembled onto the surface of the Au-deposited grassy carbon electrode. The current was proportional to chymosin activity, and thus chymosin activity could be determined. The detection ranges of chymosin activity were 2.5 to 25 U mL(-1). The detection limit of chymosin activity was 0.8 U mL(-1). The sensing platform was used to quantify chymosin activity in commercial rennet with high selectivity, excellent stability, and satisfactory reproducibility. We developed a facile, fast, and effective electrochemical assay for detecting chymosin activity, which has potential applications in cheesemaking.
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