期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 85, 期 3, 页码 277-282出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2016.04.020
关键词
Whole genome sequencing; Enterococcus faecium; Turnaround time
资金
- U.S. Army Medical Command
- Global Emerging Infections Surveillance and Response System
- Defence Medical Research and Development Program
Whole genome sequencing (WGS) is increasingly employed in clinical settings, though few assessments of turnaround times (TAT) have been performed in real-time. In this study, WGS was used to investigate an unfolding outbreak of vancomycin resistant Enterococcus faecium (VRE) among 3 patients in the ICU of a tertiary care hospital. Including overnight culturing, a TAT of just 48.5 h for a comprehensive report was achievable using an Illumina Miseq benchtop sequencer. WGS revealed that isolates from patient 2 and 3 differed from that of patient 1 by a single nucleotide polymorphism (SNP), indicating nosocomial transmission. However, the unparalleled resolution provided by WGS suggested that nosocomial transmission involved two separate events from patient 1 to patient 2 and 3, and not a linear transmission suspected by the time line. Rapid TAT's are achievable using WGS in the clinical setting and can provide an unprecedented level of resolution for outbreak investigations. Published by Elsevier Inc.
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