Combined Delivery of DOX and Kaempferol using PEGylated Gold Nanoparticles to Target Colon Cancer

Recent treatments for malignant colon cancer in clinics are not efficacious and often result in deprived patient compliance due to short therapeutic efficacy and sturdy systemic side effects. To overcome this issue in the present study, we designed and formulated nanomaterial (PEGylated AuNPs-DOX@Kaempferol) for drug delivery system against colon cancer. The drug release behavior was subsequently studied at different pH. These results showed that the drugs have been released in controlled manner. The current investigation proves that the combination of DOX and Kaempferol are more efficient to induce cytotoxic effect while compared to the each drug alone. On the other hand, AO/EtBr and DAPI staining are confirming the induction of apoptosis in cancer cells. In vivo antitumor efficiency of PEGylated AuNPs-DOX@Kaempferol showed a significant reduction in tumor volume without any severe side effects. Thus, combine drug nanoformulations could be a promising drug delivery system for colon cancer therapy.

Automated summary

A nanomaterial drug delivery system was designed for treating colon cancer, with controlled drug release behavior at different pH levels. The combination of DOX and Kaempferol was more efficient in inducing cytotoxic effects compared to each drug alone, with in vivo studies showing significant reduction in tumor volume without severe side effects.