期刊
JOURNAL OF CLINICAL VIROLOGY
卷 134, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jcv.2020.104691
关键词
Viral metagenomics; High-throughput sequencing; Next-generation sequencing; Wet lab; Recommendations
类别
Metagenomic high-throughput sequencing (mHTS) is a universal pathogen detection technique for determining DNA/RNA sequences, still in its early stages of clinical application. To support the development and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) has established the Network on Next-Generation Sequencing (ENNGS).
Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.
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