4.6 Article

Enantiomeric separation of panthenol by Capillary Electrophoresis. Analysis of commercial formulations and toxicity evaluation on non-target organisms

期刊

JOURNAL OF CHROMATOGRAPHY A
卷 1639, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.chroma.2021.461919

关键词

Capillary electrophoresis; Cyclodextrin; Chiral separation; Panthenol; Toxicity; Spirodela polyrhiza

资金

  1. Spanish Ministry of Science and Innovation [PID2019-104913GB-I00]
  2. University of Alcala [CCG19/CC068, CCG19/IA-050]
  3. Direccion General de Universidades e Investigacion de la Comunidad de Madrid (Spain) [S2018/EMT-4341]
  4. ICTS NANBIOSIS, Confocal Microscopy Service: Ciber in Bioengineering, Biomaterials & Nanomedicine (CIBER-BNN) at the Univeristy of Alcala (CAI Medicine Biology)
  5. Ministry of Science, Innovation and Universities [FPU18/00787]

向作者/读者索取更多资源

A novel CE methodology was developed for the enantiomeric separation of panthenol, with optimal conditions determined through experimental investigation. The method showed good performance for quantitating dexpanthenol in cosmetic and pharmaceutical formulations, meeting the requirements of ICH guidelines.
The first CE methodology enabling the enantiomeric separation of panthenol was developed in this work. Electrokinetic chromatography with cyclodextrins (CD-EKC) was the CE mode employed for this purpose. The effect of different experimental variables such as the nature and concentration of the cyclodextrin, the temperature and the separation voltage was investigated. The best enantiomeric separation was obtained with 25 mM (2-carboxyethyl)-beta-CD (CE-beta-CD) in 100 mM borate buffer (pH 9.0), with a separation voltage of 30 kV and a temperature of 30 degrees C. Under these conditions, an enantiomeric resolution of 2.0 in an analysis time of 4.2 min was obtained, being the biologically active enantiomer d-panthenol (dexpanthenol) the second-migrating enantiomer. The analytical characteristics of the method were evaluated in terms of precision, accuracy, selectivity, linearity, LOD, and LOQ, showing a good performance for the quantitation of dexpanthenol in cosmetic and pharmaceutical formulations. The enantiomeric impurity (L-panthenol) could be detected at a 0.1% level with respect to the majority enantiomer, allowing to accomplish the requirements of the ICH guidelines. The method was also successfully applied to study the stability of panthenol under abiotic and biotic conditions and its toxicity on non-target organisms (the aquatic plant Spirodela polyrhiza). (C) 2021 Elsevier B.V. All rights reserved.

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