期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1634, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.chroma.2020.461653
关键词
Pore size; Ion-pair RPLC; Oligonucleotides; Gradient elution; Adsorption isotherm; Surface area
资金
- Swedish Knowledge Foundation [20170059]
- Swedish Research Council (VR) [2015-04627]
- Swedish Research Council [2015-04627] Funding Source: Swedish Research Council
A combined experimental and theoretical study was performed to understand how the pore size of packing materials with pores 60-300 angstrom in size affects the separation of 5-50-mer oligonucleotides. For this purpose, we developed a model in which the solutes were described as thin rods to estimate the accessible surface area of the solute as a function of the pore size and solute size. First, an analytical investigation was conducted in which we found that the selectivity increased by a factor of 2.5 when separating 5- and 15-mer oligonucleotides using packing with 300 angstrom rather than 100 angstrom pores. We complemented the analytical investigation by theoretically demonstrating how the selectivity is dependent on the column's accessible surface area as a function of solute size. In the preparative investigation, we determined adsorption isotherms for oligonucleotides using the inverse method for separations of a 9- and a 10-mer. We found that preparative columns with a 60 angstrom-pore-size packing material provided a 10% increase in productivity as compared with a 300 A packing material, although the surface area of the 60 angstrom packing is as much as five time larger. (C) 2020 The Author(s). Published by Elsevier B.V.
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