4.7 Article

Conformational Changes of Thyroid Receptors in Response to Antagonists

期刊

JOURNAL OF CHEMICAL INFORMATION AND MODELING
卷 61, 期 2, 页码 1010-1019

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AMER CHEMICAL SOC
DOI: 10.1021/acs.jcim.0c01403

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  1. NVIDIA Corporation

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Thyroid hormone receptors (TRs) are crucial for human development, growth, and metabolism, and antagonists of TRs can effectively treat hyperthyroidism. Computational methods such as MD simulations provide valuable insights into ligand-induced conformational changes in nuclear receptors, which can be important for the development of novel therapeutics.
Thyroid hormone receptors (TRs) play a critical role in human development, growth, and metabolism. Antagonists of TRs offer an attractive strategy to treat hyperthyroidism without the disadvantage of a delayed onset of drug action. While it is challenging to examine the atomistic behavior of TRs in a laboratory setting, computational methods such as molecular dynamics (MD) simulations have proven their value to elucidate ligand-induced conformational changes in nuclear receptors. Here, we performed MD simulations of TR alpha and TR beta complexed to their native ligand triiodothyronine (T3) as well as several antagonists. Based on the examination of 27 mu s MD trajectories, we showed how binding of these compounds influences various structural features of the receptors including the helicity of helices 3 and 10 as well as the location of helix-12. Helices 3 and 12 are known to mediate coactivator association required for downstream signaling, suggesting these changes to be the molecular basis for TR antagonism. A mechanistic analysis of the trajectories revealed an allosteric pathway between H3 and H12 to be responsible for the conformational adaptations. Even though a mechanistic understanding of conformational adaptations triggered by TR antagonists is important for the development of novel therapeutics, they have not been previously examined in detail as it was done here.

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