期刊
JOURNAL OF CELLULAR PHYSIOLOGY
卷 236, 期 7, 页码 5080-5097出版社
WILEY
DOI: 10.1002/jcp.30216
关键词
fructose metabolism; Heterocephalus glaber; metabolic rate depression; miRNA; OxymiR
资金
- Natural Sciences and Engineering Research Council of Canada [04229-2015, 6793]
The study revealed significant differential expression of miRNAs in naked mole-rat brains during hypoxia, primarily by suppressing energy-expensive processes to adapt to oxygen-deprived environments. Additionally, miRNAs were found to coordinate anaerobic glycolysis and induce neuroprotective, neuroinflammatory, and central signal transduction pathways during hypoxia.
Naked mole-rats are among the mammalian champions of hypoxia tolerance. They evolved adaptations centered around reducing metabolic rate to overcome the challenges experienced in their underground burrows. In this study, we used next-generation sequencing to investigate one of the factors likely supporting hypoxia tolerance in naked mole-rat brains, posttranscriptional microRNAs (miRNAs). Of the 212 conserved miRNAs identified using small RNA sequencing, 18 displayed significant differential expression during hypoxia. Bioinformatic enrichment revealed that hypoxia-mediated miRNAs were suppressing energy expensive processes including de novo protein translation and cellular proliferation. This suppression occurred alongside the activation of neuroprotective and neuroinflammatory pathways, and the induction of central signal transduction pathways including HIF-1 alpha and NF kappa B via miR-335, miR-101, and miR-155. MiRNAs also coordinated anaerobic glycolytic fuel sources, where hypoxia-upregulated miR-365 likely suppressed protein levels of ketohexokinase, the enzyme responsible for catalyzing the first committed step of fructose catabolism. This was further supported by a hypoxia-mediated reduction in glucose transporter 5 proteins that import fructose into the cell. Yet, messenger RNA and protein levels of lactate dehydrogenase, which converts pyruvate to lactate in the absence of oxygen, were elevated during hypoxia. Together, this demonstrated the induction of anaerobic glycolysis despite a lack of reliance on fructose as the primary fuel source, suggesting that hypoxic brains are metabolically different than anoxic naked mole-rat brains that were previously found to shift to fructose-based glycolysis. Our findings contribute to the growing body of oxygen-responsive miRNAs OxymiRs that facilitate natural miRNA-mediated mechanisms for successful hypoxic exposures.
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