期刊
JOURNAL OF CELLULAR PHYSIOLOGY
卷 236, 期 6, 页码 4681-4693出版社
WILEY
DOI: 10.1002/jcp.30190
关键词
Ca2+ translocating channelrhodopsin; Ca2+‐ dependent transcription factors; cell migration; channelrhodopsin‐ 2; optogenetics
资金
- Ministry of Science and Technology [109-2320-B-006-037, 109-2628-B-006-012]
This study demonstrates the integral role of Ca2+ signals in cell biology, and optogenetic technology can be used to modulate Ca2+ waves and activate specific signaling pathways to explore the regulation of cell migration.
The ability of a single Ca2+ ion to play an important role in cell biology is highlighted by the need for cells to form Ca2+ signals in the dimensions of space, time, and amplitude. Thus, spatial and temporal changes in intracellular Ca2+ concentration are important for determining cell fate. Optogenetic technology has been developed to provide more precise and targeted stimulation of cells. Here, U2OS cells overexpressing Ca2+ translocating channelrhodopsin (CatCh) were used to mediate Ca2+ influx through blue light illumination with various parameters, such as intensity, frequency, duty cycle, and duration. We identified that several Ca2+-dependent transcription factors and certain kinases can be activated by specific Ca2+ waves. Using a wound-healing assay, we found that low-frequency Ca2+ oscillations increased cell migration through the activation of NF-kappa B. This study explores the regulation of cell migration by Ca2+ signals. Thus, we can choose optical parameters to modulate Ca2+ waves and achieve activation of specific signaling pathways. This novel methodology can be applied to clarify related cell-signaling mechanisms in the future.
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