期刊
JOURNAL OF CELLULAR PHYSIOLOGY
卷 236, 期 8, 页码 6042-6054出版社
WILEY
DOI: 10.1002/jcp.30290
关键词
bone marrow; Chemerin; osteogenesis; osteogenic differentiation
资金
- National Key R&D Program of China [2018YFA0800400]
- National Natural Science Foundation of China, China [81770861, 31571401, 82070899]
- Chongqing Science and Technology Foundation, China [cstc2018jcyjAX0232]
- Science and Technology Research Program of Chongqing Municipal Education Commission [KJZD-K201800402]
The study revealed the role of chemerin in bone metabolism, demonstrating its ability to promote osteogenic differentiation and bone formation. This provides a new theoretical basis for chemerin as a therapeutic strategy for osteoporosis.
Chemerin, a secreted protein mainly produced by adipocytes and hepatocytes, plays a variety of roles in endocrine or paracrine signaling. As reported in human epidemiology, chemerin was correlated with osteoporosis. And the previous in vitro study found that chemerin knockdown promoted osteogenesis and inhibited adipogenesis. However, the function of chemerin in bone metabolism and the underlying mechanism remains unclear. In this study, we uncovered the in vivo function of chemerin in bone homeostasis. We discovered that in obese mice, chemerin was increased in serum, while decreased in the bone marrow; and the chemerin expression in bone tissue was positively correlated with osteogenic genes. To further investigate the function of chemerin in bone metabolism, we generated chemerin deficiency and overexpression mice. We found bone mass and osteogenesis were decreased in chemerin deficiency mice, while were increased in chemerin overexpression mice. Furthermore, we observed that the chemerin expression increased during osteogenic differentiation of MSCs. Besides, we verified that chemerin promoted osteogenic differentiation in C3H10T1/2 cells and BMSCs through Akt/Gsk3 beta/beta-catenin axis. Treatment with Akt inhibitor (MK2206) abolished the promoting effect of chemerin on osteogenic differentiation and active beta-catenin. Together, our results suggest chemerin in bone marrow, not in serum, promotes osteogenic differentiation and bone formation via Akt/Gsk3 beta/beta-catenin axis. Chemerin may serve as a therapeutic strategy for osteoporosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据