A novel miRNA inhibits metastasis of prostate cancer via decreasing CREBBP-mediated histone acetylation

Background To identify novel miRNAs implicated in prostate cancer metastasis. Methods Sixty-five prostate cancer tissues and paired pan-cancer tissues were sequenced. Novel miRNAs were re-analyzed by MIREAP program. Biological functions of miR-N5 were transwell experiment and colony formation. Target genes of miR-N5 were analyzed by bioinformatic analysis. Downstream of target gene was analyzed by The Cancer Genome Atlas (TCGA) and Memorial Sloan Kettering Cancer Center (MSKCC) databases and confirmed by CHIP experiment. Results We identified a novel miRNA-miR-N5, which was downregulated in PCa cells, PCa tissue, and in the serum of patients with PCa. Knockout of miR-N5 enhanced migration and invasiveness in vitro. miR-N5 specified targeted CREBBP 3 '-UTR and inhibited CREBBP expression, which mediated H3K56 acetylation at the promoter of EGFR, beta-catenin and CDH1. Conclusion This study may shed the light on miR-N5 which influences metastasis via histone acetylation.

Automated summary

This study identified a novel miRNA, miR-N5, that was downregulated in PCa cells, tissues, and serum of PCa patients. Knockout of miR-N5 enhanced migration and invasiveness in vitro. miR-N5 targeted CREBBP 3'-UTR and inhibited CREBBP expression, which mediated H3K56 acetylation at the promoters of EGFR, beta-catenin, and CDH1.