Biocompatibility of magnetic nanoparticles coating with polycations using A549 cells

Fe3O4 nanoparticles were obtained by chemical coprecipitation of iron chloride and sodium hydroxide. The morphology and sizes of the obtained nanoparticles were characterized using laser Doppler velocimetry, transmission electron and atomic force microscopy. Then the nanoparticles were stabilized by three polycations (polyethylenimine (PEI), poly(allylamine hydrochloride) (PAH), poly(diallyldimethylammonium chloride) (PDADMAC)) to increase their biocompatibility. The cytotoxicity of the obtained polymer-stabilized nanoparticles was studied using a human lung carcinoma cell line (A549). The biodistribution of nanoparticles stabilized by polycations in human lung carcinoma cells was analyzed by transmission electron microscopy, and the toxicity of nanomaterials was evaluated using toxicity tests and flow cytometry. As a result, the most biocompatible nanoparticle-biopolymer complex was identified. PAH stabilized magnetic nanoparticles demonstrated the best biocompatibility, and the PEI-magnetic nanoparticle complex was the most toxic.

Automated summary

Fe3O4 nanoparticles were synthesized by chemical coprecipitation of iron chloride and sodium hydroxide. Stabilized by different polycations, the biocompatibility and toxicity of these nanoparticles in human lung carcinoma cells were studied, with PAH-stabilized magnetic nanoparticles showing the best biocompatibility and the PEI-magnetic nanoparticle complex being the most toxic.