4.7 Article

Characterization of human prostasomes protein Clusterin (macromolecule) - a novel biomarker for male infertility diagnosis and prognosis

期刊

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
卷 40, 期 9, 页码 3979-3988

出版社

TAYLOR & FRANCIS INC
DOI: 10.1080/07391102.2020.1852960

关键词

Male infertility; prostasomes; Clusterin; threading; molecular dynamics simulation

资金

  1. DST-SERB Research grant under Young Scientist scheme

向作者/读者索取更多资源

This study characterized prostasomes using biochemical, molecular, and in silico methods, and identified a novel protein biomarker Clusterin for the diagnosis and prognosis of male infertility.
Prostasomes, a secretory particle from prostate gland in human seminal fluid plays a role in enhancing the fertility and its absence or less presence will lead to male infertility. Very few fertility associated proteins were detected in prostasomes. In order to isolate the prostasomes from ejaculated semen, the characterization was done using biochemical, molecular and in silico methods. The objective of current work on prostasomes is to identify a novel protein biomarker for the diagnosis and prognosis of male infertility. Semen samples were collected, primary semen analysis was done and prostasomes were isolated from ejaculated semen of fertile and infertile categories. Biochemical parameters like protein content, total antioxidant capacity, cholesterol content were evaluated in prostasomes. The critical expressed protein was identified by using SDS page and MALDI TOF techniques. Isolated particle from the semen samples was confirmed as prostasomes from SEM analysis and particle size analyzer. In MALDI results, the maximum hit was obtained against Clusterin that been reported to be involved in capacitation and motility of sperm. Structural modeling and molecular dynamics were carried out on Clusterin and elucidate the structural insights in the dynamic system. Overall, our study reported the novel biomarker Clusterin in prostasomes for diagnosis and prognosis of male infertility. Communicated by Ramaswamy H. Sarma

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据