期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
卷 109, 期 9, 页码 1244-1258出版社
WILEY
DOI: 10.1002/jbm.b.34785
关键词
apical papilla cells; fibronectin; nanofibers; pulp regeneration; tissue engineering
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [302047/2019-0, 408721/2018-9]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [2017/14210-8, 2017/22739-9]
Fibronectin-loaded nanofiber scaffolds were developed and evaluated for their bioactive potential on human apical papilla cells. Both random (NR) and aligned (NA) scaffolds showed good cytocompatibility, with NR and NA based on 10% PCL promoting the highest cell proliferation, adhesion, and spreading. Increasing the concentration of fibronectin led to enhanced cell migration, viability, proliferation, adhesion, collagen synthesis, and gene expression. Additionally, NA tubular scaffolds loaded with 10 µg/ml fibronectin exhibited the highest chemotactic potential on human apical papilla cells.
Fibronectin (FN)-loaded nanofiber scaffolds were developed and assessed concerning their bioactive potential on human apical papilla cells (hAPCs). First, random (NR) and aligned (NA) nanofiber scaffolds of polycaprolactone (PCL) were obtained by electrospinning technique and their biological properties were evaluated. The best formulations of NR and NA were loaded with 0, 5, or 10 mu g/ml of FN and their bioactivity was assessed. Finally, FN-loaded NR and NA tubular scaffolds were prepared and their chemotactic potential was analyzed using an in vitro model to mimic the pulp regeneration of teeth with incomplete root formation. All scaffolds tested were cytocompatible. However, NR and NA based on 10% PCL promoted the highest hAPCs proliferation, adhesion and spreading. Polygonal and elongated cells were observed on NR and NA, respectively. The higher the concentration of FN added to the scaffolds, greater cell migration, viability, proliferation, adhesion and spreading, as well as collagen synthesis and gene expression (ITGA5, ITGAV, COL1A1, COL3A1). In addition, tubular scaffolds with NA loaded with FN (10 mu g/ml) showed the highest chemotactic potential on hAPCs. It was concluded that FN-loaded NA scaffolds may be an interesting biomaterial to promote hAPCs-mediated pulp regeneration of endodontically compromised teeth with incomplete root formation.
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