期刊
JOURNAL OF BIOMECHANICS
卷 114, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jbiomech.2020.110159
关键词
Collagen; Raman spectroscopy; Glycosaminoglycans; Integration; Biomechanics; Composition; Elastography
资金
- NIH [R01AR071394-01]
- NSF CMMI [1536463]
- Department of Education (GAANN Fellowship) [P200A150273 J]
- NSF MRSEC program [DMR-1719875]
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) of the National Institutes of Health (NIH) [F31AR070009]
- Div Of Civil, Mechanical, & Manufact Inn
- Directorate For Engineering [1536463] Funding Source: National Science Foundation
The study found that the interface slope had a stronger correlation with local tissue strains under compressive and shear loading, compared to current histological integration assessments and local compositional measures. Therefore, controlling interface geometry at the time of surgery is important for cartilage repair integration and long-term healing.
Achieving lateral integration of articular cartilage repair tissue with surrounding native cartilage remains a clinical challenge. Histological and bulk mechanical studies have identified extracellular matrix components that correlate with superior failure strength, but it is unclear how local changes in geometry and composition at the repair interface affect tissue strains under physiologic loading. Here, we investigated the effects of local compositional and interface geometry on lateral cartilage repair integration by coupling microscale Raman spectroscopy and confocal elastography to measure tissue strains under compressive and shear loading. Histological integration assessments did not have significant relationships with interface strains under compressive loading (p > 0.083) and only the perimeter attachment score was trending towards statistical significance with the vertical bar E-xy vertical bar strain tensor under shear loading (p = 0.050). Interface slope had a stronger correlation with local tissue strains under compressive and shear loading compared to compositional measures of GAG, collagen, or proteins (compressive loading vertical bar E-xy vertical bar tensor: R-2 = 0.400 (interface slope), 0.005 (GAG), 0.024 (collagen), and 0.012 (protein); shear loading vertical bar E-xy vertical bar tensor: R-2 = 0.457 (interface slope), 0.003 (GAG), 0.006 (collagen), and 0.000 (total protein)). These data support surgical publications detailing the need for vertical walls when debriding chondral defects. Current histological integration assessments and local compositional measures were insufficient for identifying the variation in interface strains under compressive and shear loading. Thus, our data points to the importance of controlling interface geometry at the time of surgery, which has implications for cartilage repair integration and long-term healing. (C) 2020 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据