期刊
JOURNAL OF BIOCHEMISTRY
卷 169, 期 5, 页码 601-611出版社
OXFORD UNIV PRESS
DOI: 10.1093/jb/mvaa148
关键词
circGFRA1; miR-361-5p; PTX; TLR4; triple-negative breast cancerNatural Science Foundation of Zhejiang Province of China [LY19H160026], Zhejiang Provincial Health Department [2019KY454], Wenzhou Bureau of Science and Technology [Y20170038, Y20170257]
资金
- Natural Science Foundation of Zhejiang Province of China [LY19H160026]
- Zhejiang Provincial Health Department [2019KY454]
- Wenzhou Bureau of Science and Technology [Y20170038, Y20170257]
This study clarifies that circGFRA1 affects the sensitivity of TNBC cells to PTX via the miR-361-5p/TLR4 pathway. Knockdown of circGFRA1 can inhibit TNBC cell resistance to PTX by promoting the expression of miR-361-5p and subsequently reducing the expression of TLR4.
In recent years, the role of circular RNAs (circRNAs) in tumours has attracted widespread attention. Some circRNAs have been reported to play a role in triple-negative breast cancer (TNBC). However, circRNAs have rarely been reported in terms of TNBC resistance. This study aimed to clarify that circGFRA1 affects the sensitivity of TNBC cells to paclitaxel (PTX) by the miR-361-5p/TLR4 pathway. Compared with the non-PTX-resistant TNBC cell line MDA-MB-231, the expression of circGFRA1 in the PTX-resistant TNBC cell line MDA-MB-231.PR was significantly increased. The small hairpin RNA-mediated circGFRA1 knockdown inhibited the resistance of TNBC cells to PTX. RNA pull-down assay and luciferase reporter gene assay confirmed the binding between circGFRA1 and miR-361-5p and between miR-361-5p and TLR4. It has been proven that circGFRA1 knockdown can inhibit the resistance of TNBC cells to PTX by promoting the expression of miR-361-5p, and subsequently reduce the expression of TLR4.
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