期刊
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
卷 38, 期 1, 页码 129-138出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10815-020-02013-z
关键词
Male reciprocal translocation; Sperm FISH; ICSI; Quadrivalent structure; Segregation patterns; Alternate products
资金
- Science and Technology Innovation Action Plan Program of Shanghai [18411953800]
- Shanghai Shen Kang Hospital Development Centre Municipal Hospital New Frontier Technology Joint Project [SHDC12017105]
The study evaluated the chromosome contents of selected sperm for ICSI from male carriers of reciprocal translocation, finding higher rates of normal/balanced chromosome contents compared to unselected sperm. ICSI improved embryo rates and euploidy in male carriers, showing significant differences in chromosome contents between sperm and embryos from male carriers.
Purpose To evaluate whether the morphologically normal spermatozoa selected for intracytoplasmic sperm injection (ICSI) under microscope had a higher rate of normal/balanced chromosome contents than that in the whole unselected sperm from reciprocal translocation carriers. Methods Five hundred unselected spermatozoa from each of 40 male translocation carriers were performed with fluorescence in situ hybridization (FISH), to determine the rates of gametes with different meiotic contents of translocated chromosomes. Meanwhile, 3030 biopsied blastocysts from 239 male and 293 female reciprocal translocation carriers were detected with the microarray technique to analyze the rates of embryos with different translocated chromosome contents. Results The D3 embryo rate, blastocyst formation rate, and euploid rate of blastocysts were remarkably higher in male carriers than those in female (p = 0.001, p = 0.004, and p = 0.035, respectively). In addition, the percentages of alternate products, which contained normal/balanced chromosome contents, in embryos from male carriers were markedly higher than those in sperm FISH (p = 2.48 x 10(-5) and p = 2.88 x 10(-10)), while the percentages of adjacent-2 and 3:1 products were lower than those in sperm FISH (p = 0.003 and p = 5.28 x 10(-44)). Moreover, consistent results were obtained when comparing the rates of products in embryos between male and female carriers. Specifically, the incidence of alternate products in male carriers was higher than those in female carriers (p = 0.022). However, no similar differences were seen between sperm and embryos of female carriers. Conclusion ICSI facilitates the selection of spermatozoa with normal/balanced chromosome contents and improves the D3 embryo rate, blastocyst formation rate, and the euploid embryo rate in male carriers.
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