期刊
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
卷 38, 期 2, 页码 443-459出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10815-020-02010-2
关键词
Melatonin; miRNA expression; Cell-free DNA; Follicular fluid; Embryo quality; Unexplained infertility
The study found that patients with low melatonin levels in follicular fluid had significantly higher oxidative stress markers, cfDNA levels, and lower relative expression of certain miRNAs compared to other groups. These patients also had lower fertilization rates and fewer high-quality day 3 embryos.
Purpose Intrafollicular fluid (IFF) melatonin plays a decisive role in maintaining granulosa cells' DNA integrity and protects them against apoptosis. It reduces oxidative stress and improves the oocyte quality with a higher fertilization rate. Method This prospective study investigated the antioxidant property of IFF melatonin and its impact on IVF outcome parameters. We also explored the relative expression of five microRNAs (miR-663b, miR-320a, miR-766-3p, miR-132-3p, miR-16-5p) and levels of cell-free DNA (cfDNA) by real-time PCR in unexplained infertile patients. We collected 425 follicular fluid (FF) samples containing mature oocytes from 295 patients undergoing IVF. Results Patients were subgrouped based on IFF melatonin concentration (group A <= 30 pg/mL, group B > 70 to <= 110 pg/mL, group C > 111 to <= 385 pg/mL). Our results showed that patients with <= 30 pg/mL IFF melatonin levels have significantly higher oxidative stress markers, cfDNA levels, and lower relative expression of miR-663b, miR-320a, miR-766-3p, miR-132-3p, and miR-16-5p compared to other subgroups (p < 0.001). Similarly, they have a low fertilization rate and a reduced number of high-quality day 3 embryos. Conclusion Findings suggest that the therapeutic use of melatonin produces a considerable rise in the number of mature oocytes retrieved, fertilization rate, and good-quality embryo selection. Furthermore, miRNA signature enhances the quality of embryo selection, thus, may allow us to classify them as non-invasive biomarkers to identify good-quality embryos.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据