期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 68, 期 52, 页码 15478-15489出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c05028
关键词
rhamnolipid; antibiofilm; characterization; foodborne pathogen; LC-MS
资金
- National Natural Science Foundation of China [31972042, 31950410550]
- National Key R&D Program of China [2017YFC1600806]
- China Postdoctoral Science Foundation [2019M661759]
- Postdoctoral Science Foundation of Jiangsu Province, China [2020Z154]
- High-Level Talents Project of Six Talent Peaks in Jiangsu Province of China [SWYY-018]
Rhamnolipid is the main group of biosurfactants predominantly produced by Pseudomonas aeruginosa, a ubiquitous and opportunistic pathogen, which limits its large-scale exploitation. Thus, cost-effective rhamnolipid production from a newly isolated nonpathogenic Enterobacter sp. UJS-RC was investigated. The highest rhamnolipid production (4.4 +/- 0.2 g/L) was achieved in a medium constituting agroindustrial wastes (sugarcane molasses and corn steep liquor) as substrates. Rhamnolipid exhibited reduced surface tension to 72-28 mN/m with an emulsification index of 75%. The structural analyses demonstrated the presence of methoxyl, carboxyl, and hydroxyl groups in rhamnolipid. Mass spectra indicated eight rhamnolipid congeners, where dirhamnolipid (m/z 650.01) was the dominant congener. Rhamnolipid inhibited biofilm formation of Staphylococcus aureus in a dose-dependent manner, supported by scanning electron microscopy disclosing the disruption of the microcolony/exopolysaccharide matrix. Rhamnolipid's ability to generate reactive oxygen species has thrown light on the mechanism through which the killing of test bacteria may occur.
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