期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 1, 页码 275-285出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c06504
关键词
Yarrowia lipolytica; alpha-pinene; waste cooking oil; lignocellulosic hydrolysate
资金
- National Key R&D Program of China [2019YFA0904302]
- National Natural Science Foundation of China [21776081]
- Natural Science Foundation of Shanghai [18ZR1410000]
The study engineered an orthogonal biosynthetic pathway in oleaginous yeast for efficient production of alpha-pinene, achieving high titers using waste cooking oil and lignocellulosic hydrolysate as carbon sources.
alpha-Pinene, an important biologically active natural monoterpene, has been widely used in fragrances, medicines, and fine chemicals, especially, in high-density renewable fuels such as jet fuel. The development of an alpha-pinene production platform in a highly modifiable microbe from renewable substitute feedstocks could lead to a green, economical avenue, and sustainable biotechnological process for the biosynthesis of alpha-pinene. Here, we report engineering of an orthogonal biosynthetic pathway for efficient production of alpha-pinene in oleaginous yeast Yarrowia lipolytica that resulted in an alpha-pinene titer of 19.6 mg/L when using glucose as the sole carbon source, a significant 218-fold improvement than the initial titer. In addition, the potential of using waste cooking oil and lignocellulosic hydrolysate as carbon sources for alpha-pinene production from the engineered Y. lipolytica strains was analyzed. The results indicated that alpha-pinene titers of 33.8 and 36.1 mg/L were successfully obtained in waste cooking oil and lignocellulosic hydrolysate medium, thereby representing the highest titer reported to date in yeast. To our knowledge, this is also the first report related to microbial production of alpha-pinene from waste cooking oil and lignocellulosic hydrolysate.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据