4.7 Article

Preparation of Boronic Acid-Functionalized Cryogels Using Modular and Clickable Building Blocks for Bacterial Separation

期刊

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 1, 页码 135-145

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c06052

关键词

composite cryogel; reversible addition fragmentation chain transfer polymerization; boronate affinity; bacteria; bioseparation

资金

  1. Swedish Research Council VR [2019-04228]
  2. China Scholarship Council (CSC)
  3. Swedish Research Council [2019-04228] Funding Source: Swedish Research Council

向作者/读者索取更多资源

Composite cryogels containing boronic acid ligands were synthesized for efficient separation and isolation of bacteria. The bacterial binding is controlled through reversible boronate ester bonds, which can be regulated by pH and simple monosaccharides. The composite cryogel shows promising potential as an affinity medium for rapid separation and isolation of bacteria.
Composite cryogels containing boronic acid ligands are synthesized for effective separation and isolation of bacteria. The large and interconnected pores in cryogels enable fast binding and release of microbial cells. To control bacterial binding, an alkyne-tagged boronic acid ligand is conjugated to azide-functionalized cryogel via the Cu(I)-catalyzed azide-alkyne cycloaddition reaction. The boronic acid-functionalized cryogel binds Gram-positive and Gram-negative bacteria through reversible boronate ester bonds, which can be controlled by pH and simple monosaccharides. To increase the capacity of affinity separation, a new approach is used to couple the allcyne-tagged phenylboronic acid to cryogel via an intermediate polymer layer that provides multiple immobilization sites. The morphology and chemical composition of the composite cryogel are characterized systematically. The capability of the composite cryogel for the separation of Gram-positive and Gram-negative bacteria is investigated. The binding capacities of the composite cryogel for Escherichia coli and Staphylococcus epidermidis are 2.15 x 10(9) and 3.36 x 10(9) cfu/g, respectively. The bacterial binding of the composite cryogel can be controlled by adjusting pH. The results suggest that the composite cryogel may be used as affinity medium for rapid separation and isolation of bacteria from complex samples.

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