期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 68, 期 47, 页码 14071-14080出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c05344
关键词
tea leaves; oolong tea; accumulation of amino acids; bruising and withering treatment; hub genes; expression regulation
资金
- National Natural Science Foundation of China [31770728]
- Major Project of Agricultural Science and Technology in Breeding of Tea Plant in Zhejiang Province [2016C02053-7]
- China Agriculture Research System (Tea) [CARS-19]
Amino acids are very important for oolong tea brisk-smooth mouthfeel which is mainly associated with bruising and withering treatment (BWT). In this study, metabolome and transcriptome analyses were performed to comprehensively investigate the changes in abundance of amino acids and the expression pattern of relevant genes during BWT of oolong tea manufacturing. Levels of most amino acids increased during BWT in the leaves harvested from 4 cultivars, while expression of the relevant function genes responsible for synthesis and transformation of amino acids up-regulated accordingly. Upstream hub genes including receptorlike protein kinase iKU2, serine/threonine-protein kinase PBL11, MTh transcription factor MYB2, ethylene-responsive transcription factor ERF114, WRKY transcription factor WRKY71, aspartate aminotransferase AATC, UDP-glycosyltransferase U91D1, and 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 RRAA2, were predicted to be involved in regulation of the function genes expression and the amino acids metabolism through weighted gene coexpression network analysis. A modulation mechanism for accumulation of amino acids during BWT was also proposed. These findings give a deep insight into the metabolic reprogramming mechanism of amino acids during BWT of oolong tea.
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