期刊
JAPANESE JOURNAL OF INFECTIOUS DISEASES
卷 74, 期 4, 页码 285-292出版社
NATL INST INFECTIOUS DISEASES
DOI: 10.7883/yoken.JJID.2020.776
关键词
-
资金
- Health, Labour, and Welfare Policy Research Grant for Special Research
The study aimed to isolate seasonal coronaviruses from human airway epithelium using modified culture methods. The results showed that ALI culture was effective in isolating HCoVs, particularly in obtaining HCoV-OC43 and HCoV-NL63. Furthermore, the LLC-MK2 cell line in combination with ALI cultures was successful in large-scale culturing of HCoV-NL63.
Isolation of seasonal coronaviruses, which include human coronavirus (HCoV) OC43, HCoV-HKU1, and HCoV-NL63, from primary cultures is difficult because it requires experienced handling, an exception being HCoV-229E, which can be isolated using cell lines such as RD-18S and HeLa-ACE2-TMPRSS2. We aimed to isolate seasonal CoVs in Yamagata, Japan to obtain infective virions useful for further research and to accelerate fundamental studies on HCoVs and SARS-CoV-2. Using modified air-liquid interface (ALI) culture of the normal human airway epithelium from earlier studies, we isolated 29 HCoVs (80.6%: 16, 6, 6, and 1 isolates of HCoV-OC43, HCoV-HKU1, HCoVNL63, and HCoV-229E, respectively) from 36 cryopreserved nasopharyngeal specimens. In ALI cultures of HCoV-OC43 and HCoV-NL63, the harvested medium contained more than 1 x 104 genome copies/4 at every tested time point during the more than 100 days of culture. Four isolates of HCoVNL63 were further subcultured and successfully propagated in an LLC-MK2 cell line. Our results suggest that ALI culture is useful for isolating seasonal CoVs and sustainably obtaining HCoV-OC43 and HCoV-NL63 virions. Furthermore, the LLC-MK2 cell line in combination with ALI cultures can be used for the large-scale culturing of HCoV-NL63. Further investigations are necessary to develop methods for culturing difficult-to-culture seasonal CoVs in cell lines.
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