Aeromicrobium terrae sp. nov., isolated from a maize field

A polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium, designated strain CC-CFT486(T), isolated from soil sampled in a maize field in Taiwan. Cells of strain CC-CFT486(T) were short rods, motile with polar flagella, catalase-positive and oxidase-positive. Optimal growth occurred at 30 degrees C, pH 8 and 1 % NaCl. Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486(T) associated with Aeromicrobium panacisoli (97.0% sequence identity), Aeromicrobium lacus (97.0%), Aeromicrobium erythreum (96.8%) and Aeromicrobium alkaliterrae (96.8%), and lower sequence similarity values to other species. Average nucleotide identity (ANI) values were 70.6-77.8% (n=11) compared within the type strains of the genus Aeromicrobium. Strain CC-CFT486(T) contained C-16:0, C-17:0, C-17:1 omega 8c and C-18:1 omega 9c as the predominant fatty acids. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, two unidentified aminophospholipids and three unknown phospholipids. The cell wall peptidoglycan of strains CC-CFT486(T) contained LL-diaminopimelic acid (LL-DAP) and the major polyamine was spermidine. The DNA G+C content was 70.6 mol% and the predominant quinone was menaquinone 9 (MK-9). Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486(T) is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp. nov. (type strain CC-CFT486(T)=BCRC 81217(T)=JCM 33499(T)).

Automated summary

In this study, a polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium isolated from soil in a maize field in Taiwan, which was named Aeromicrobium terrae. Analyses based on phylogeny, phenotypic traits, chemotaxonomic characteristics, 16S rRNA gene sequences, and ANI values supported the proposal of this bacterium as a novel species within the genus Aeromicrobium.