期刊
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
卷 27, 期 2, 页码 1197-1203出版社
SPRINGER
DOI: 10.1007/s10989-021-10160-9
关键词
Lipopeptide biosurfactant; Acinetobacter junii; Angiogenesis; Wound healing
资金
- Kerman University of Medical Sciences (KMU) [97000960]
Lipopeptide biosurfactants (LPBs) were studied for their effect on human umbilical vein endothelial cells (HUVECs), showing no significant proliferative effect but an increase in migration and tube formation rates at a concentration of 300 μg/mL, along with enhanced expression of angiogenic factors.
Lipopeptide biosurfactants (LPBs) display unique properties with widespread therapeutic applications. Recently, the wound healing activity of LPBs has received more attention. However, few investigations have focused on the healing mechanisms of these compounds, particularly those that are associated with the angiogenic pathways. Therefore, the current study aimed at investigating the effect of a natural LPB produced by Acinetobacter. junii B6 on the angiogenic potential of human umbilical vein endothelial cells (HUVECs). The proliferation, migration, and three-dimensional tube forming potential of HUVECs were examined after treatment with LPB. By using western blotting, the expression of angiogenic-related genes such as hypoxia-inducible factor-1 alpha (HIF-1 alpha) and vascular endothelial growth factor (VEGF) were also measured. LPB demonstrated no significant proliferative or cytotoxic effect on HUVECs at the concentrations lower than 600 mu g/mL. Moreover, LPB at a concentration of 300 mu g/mL caused a significant increase in the rates of migration and tube formation of HUVECs. LPB also enhanced the protein expression levels of HIF-1 alpha and VEGF in HUVECs in a dose-dependent manner. The present results suggest that LPB might be considered a potential wound healing agent by modifying some angiogenic factors.
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