期刊
INTERNATIONAL JOURNAL OF NANOMEDICINE
卷 16, 期 -, 页码 421-432出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S286474
关键词
nanodroplets; chitosan; DKK-2; ultrasonic gene transfer; prostate cancer
资金
- Natural Science Foundation of Shandong Province of China [ZR2019PH117]
- National Natural Science Foundation of China [81771843]
- Science and Technology Developing Program of Shandong Provincial Government of China [2017GSF18107]
The study synthesized echogenic chitosan/perfluorohexane nanodroplets (CNDs) and demonstrated their potential for gene delivery, showing promising applications in ultrasound-mediated gene delivery and cell therapy.
Purpose: To synthesize echogenic chitosan/perfluorohexane nanodroplets (CNDs) for DKK-2 gene delivering in a spatiotemporally controlled manner in vitro. Methods: The characteristics, contrast-enhanced ultrasound imaging, DNA binding and DNase protection capacity, DKK-2 gene transfection and effects on LNCaP cells of these CNDs were investigated. Results: The obtained CNDs showed positive surface charges and could attract the genetic cargo with negative surface charges to form nanocomplexes. Agarose gel electrophoresis confirmed binding of the CNDs and pDNA. DKK-2 pDNA-loaded CNDs, in combination with ultrasound, ruptured and released DKK-2 pDNA, entering LNCaP cells through nano-scale pores in the cell membrane, which further reduced the proliferation of LNCaP cells. Conclusion: These stable and safe CNDs may be a promising choice to achieve efficient ultrasound-mediated gene delivery to specific tissues in a spatiotemporally controlled manner.
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