期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/ijms22020674
关键词
tissue engineering; nerve repair; nerve tissue decellularization; genipin; chemical crosslinking; histology; natural biomaterials; biomechanical and structural properties; cell-biomaterials interactions
资金
- Spanish Plan Nacional de Investigacion Cientifica, Desarrollo e Innovacion Tecnologica, Ministerio de Economia y Competitividad (Instituto de Salud Carlos III) [FIS PI14-1343, FIS PI17-0393, FIS PI20-0318]
- Fondo Europeo de Desarrollo Regional ERDF-FEDER European Union
- Plan Andaluz de Investigacion, Desarrollo e Innovacion (PAIDI 2020), Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades, Junta de Andalucia, Espana [P18-RT-5059]
- Programa Operativo FEDER Andalucia 2014-2020, Universidad de Granada, Junta de Andalucia, Espana [A-CTS-498UGR18]
- ERDF-FEDER, the European Union
Acellular nerve allografts (ANGs) have shown to be a promising alternative in nerve repair. This study demonstrates that using genipin (GP) as a crosslinking agent can improve the biomechanical properties of ANGs, especially in RS and RS-GP 0.10% ANGs, with minimal impact on biocompatibility and histological pattern. This novel crosslinked ANGs could be a suitable alternative for future in vivo preclinical studies.
Acellular nerve allografts (ANGs) represent a promising alternative in nerve repair. Our aim is to improve the structural and biomechanical properties of biocompatible Sondell (SD) and Roosens (RS) based ANGs using genipin (GP) as a crosslinker agent ex vivo. The impact of two concentrations of GP (0.10% and 0.25%) on Wistar rat sciatic nerve-derived ANGs was assessed at the histological, biomechanical, and biocompatibility levels. Histology confirmed the differences between SD and RS procedures, but not remarkable changes were induced by GP, which helped to preserve the nerve histological pattern. Tensile test revealed that GP enhanced the biomechanical properties of SD and RS ANGs, being the crosslinked RS ANGs more comparable to the native nerves used as control. The evaluation of the ANGs biocompatibility conducted with adipose-derived mesenchymal stem cells cultured within the ANGs confirmed a high degree of biocompatibility in all ANGs, especially in RS and RS-GP 0.10% ANGs. Finally, this study demonstrates that the use of GP could be an efficient alternative to improve the biomechanical properties of ANGs with a slight impact on the biocompatibility and histological pattern. For these reasons, we hypothesize that our novel crosslinked ANGs could be a suitable alternative for future in vivo preclinical studies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据