期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/ijms22010233
关键词
osteoporosis; natural plant; loganic acid; osteoblast; osteoclast; bone mineral density; ovariectomized mice
资金
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) [116162-02-2-SB010]
- National Research Foundation of Korea (NRF) - Korean government [2020R1I1A1A01072353, 2020R1F1A1075524]
- National Research Foundation of Korea [2020R1I1A1A01072353, 2020R1F1A1075524] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Loganic acid extracted from Gentiana lutea L. root extract has shown anti-osteoporotic effects by promoting osteoblastic differentiation and inhibiting osteoclast differentiation. In vivo experiments on ovariectomized mice have demonstrated that loganic acid prevents bone mineral density loss and improves bone structural properties in osteoporotic model mice. These results suggest that loganic acid may be a potential therapeutic candidate for the treatment of osteoporosis.
Osteoporosis is a common disease caused by an imbalance of processes between bone resorption by osteoclasts and bone formation by osteoblasts in postmenopausal women. The roots of Gentiana lutea L. (GL) are reported to have beneficial effects on various human diseases related to liver functions and gastrointestinal motility, as well as on arthritis. Here, we fractionated and isolated bioactive constituent(s) responsible for anti-osteoporotic effects of GL root extract. A single phytochemical compound, loganic acid, was identified as a candidate osteoprotective agent. Its anti-osteoporotic effects were examined in vitro and in vivo. Treatment with loganic acid significantly increased osteoblastic differentiation in preosteoblast MC3T3-E1 cells by promoting alkaline phosphatase activity and increasing mRNA expression levels of bone metabolic markers such as Alpl, Bglap, and Sp7. However, loganic acid inhibited osteoclast differentiation of primary-cultured monocytes derived from mouse bone marrow. For in vivo experiments, the effect of loganic acid on ovariectomized (OVX) mice was examined for 12 weeks. Loganic acid prevented OVX-induced bone mineral density loss and improved bone structural properties in osteoporotic model mice. These results suggest that loganic acid may be a potential therapeutic candidate for treatment of osteoporosis.
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