期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 21, 期 22, 页码 -出版社
MDPI
DOI: 10.3390/ijms21228731
关键词
MHC class I; sex ratio; polyspermy block; non-random fertilization
资金
- JSPS KAKENHI [JP16KK0192, JP19H01067]
Hermaphroditic invertebrates and plants have a self-recognition system on the cell surface of sperm and eggs, which prevents their self-fusion and enhances non-self-fusion, thereby contributing to genetic variation. However, the system of sperm-egg recognition in mammals is under debate. To address this issue, we explored the role of major histocompatibility complex class I (MHC class I, also known as histocompatibility 2-K-b or H2-K-b and H2-D-b in mice) antigens by analyzing H2-K(b-/-)H2-Db-/-beta 2-microglobulin (beta 2M)(-/-) triple-knockout (T-KO) male mice with full fertility. T-KO sperm exhibited an increased sperm number in the perivitelline space of wild-type (WT) eggs in vitro. Moreover, T-KO sperm showed multiple fusion with zona pellucida (ZP)-free WT eggs, implying that the ability of polyspermy block for sperm from T-KO males was weakened in WT eggs. When T-KO male mice were intercrossed with WT female mice, the percentage of females in progeny increased. We speculate that WT eggs prefer fusion with T-KO sperm, more specifically X-chromosome-bearing sperm (X sperm), suggesting the presence of preferential (non-random) fertilization in mammals, including humans.
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