期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/ijms22031095
关键词
retinal pigment epithelium; RPE maturation; tissue-specific bioink; in vitro RPE model; tissue-mimetic substrate
资金
- National Research Foundation of Korea - Korea government (MSIP) [NRF-2019R1A3A3005437]
- Catholic University of Korea, Eunpyeong St. Mary's Hospital. Research Institute of Medical Science
- National Research Foundation of Korea [5199990614198] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The retinal pigment epithelium (RPE) plays a crucial role in retinal degenerative diseases, but there is currently no cure. Research has shown that bioink derived from porcine Bruch's membrane can enhance RPE functionality, and a Bruch's membrane-mimetic substrate (BMS) developed using 3D printing technology can facilitate appropriate RPE functions.
Retinal pigment epithelium (RPE) is a monolayer of the pigmented cells that lies on the thin extracellular matrix called Bruch's membrane. This monolayer is the main component of the outer blood-retinal barrier (BRB), which plays a multifunctional role. Due to their crucial roles, the damage of this epithelium causes a wide range of diseases related to retinal degeneration including age-related macular degeneration, retinitis pigmentosa, and Stargardt disease. Unfortunately, there is presently no cure for these diseases. Clinically implantable RPE for humans is under development, and there is no practical examination platform for drug development. Here, we developed porcine Bruch's membrane-derived bioink (BM-ECM). Compared to conventional laminin, the RPE cells on BM-ECM showed enhanced functionality of RPE. Furthermore, we developed the Bruch's membrane-mimetic substrate (BMS) via the integration of BM-ECM and 3D printing technology, which revealed structure and extracellular matrix components similar to those of natural Bruch's membrane. The developed BMS facilitated the appropriate functions of RPE, including barrier and clearance functions, the secretion of anti-angiogenic growth factors, and enzyme formation for phototransduction. Moreover, it could be used as a basement frame for RPE transplantation. We established BMS using 3D printing technology to grow RPE cells with functions that could be used for an in vitro model and RPE transplantation.
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