期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/ijms22020483
关键词
terpenoids; camphor; eucalyptol; antifungal activity; virulence factors; efflux pumps; cytotoxicity
资金
- Serbian Ministry of Education, Science and Technological Development [451-03-68/2020-14/200007]
- FEMS [FEMS-GO-2017-015]
- Foundation for Science and Technology (FCT, Portugal) through national funds FCT/MCTES [UIDB/00690/2020]
- FCT, P.I.
The study investigated the antifungal activity of camphor and eucalyptol on Candida species, revealing that camphor showed excellent antifungal properties with the potential to reduce fungal virulence traits, while eucalyptol exhibited cytotoxic effects and was deemed unsuitable as an antifungal candidate. Both compounds had different effects on efflux pump gene expression, with camphor downregulating CDR1 and eucalyptol upregulating CDR2.
Candidaalbicans represents one of the most common fungal pathogens. Due to its increasing incidence and the poor efficacy of available antifungals, finding novel antifungal molecules is of great importance. Camphor and eucalyptol are bioactive terpenoid plant constituents and their antifungal properties have been explored previously. In this study, we examined their ability to inhibit the growth of different Candida species in suspension and biofilm, to block hyphal transition along with their impact on genes encoding for efflux pumps (CDR1 and CDR2), ergosterol biosynthesis (ERG11), and cytotoxicity to primary liver cells. Camphor showed excellent antifungal activity with a minimal inhibitory concentration of 0.125-0.35 mg/mL while eucalyptol was active in the range of 2-23 mg/mL. The results showed camphor's potential to reduce fungal virulence traits, that is, biofilm establishment and hyphae formation. On the other hand, camphor and eucalyptol treatments upregulated CDR1; CDR2 was positively regulated after eucalyptol application while camphor downregulated it. Neither had an impact on ERG11 expression. The beneficial antifungal activities of camphor were achieved with an amount that was non-toxic to porcine liver cells, making it a promising antifungal compound for future development. The antifungal concentration of eucalyptol caused cytotoxic effects and increased expression of efflux pump genes, which suggests that it is an unsuitable antifungal candidate.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据