期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 47, 期 3, 页码 -出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2021.4852
关键词
aerobic glycolysis; 2-deoxy-D-glucose; autophagy; acute kidney injury; sepsis
资金
- National Natural Science Foundation of China [81871610, 81870071, 81471897, 81671895]
Inhibition of aerobic glycolysis protects against sepsis-induced AKI by promoting autophagy via the lactate/SIRT3/AMPK pathway. Sepsis or LPS enhance aerobic glycolysis, while the inhibitor 2-DG downregulates glycolysis and improves kidney injury induced by sepsis by enhancing cellular autophagy.
Metabolism reprogramming influences the severity of organ dysfunction, progression to fibrosis, and development of disease in acute kidney injury (AKI). Previously we showed that inhibition of aerobic glycolysis improved survival rates and protected septic mice from kidney injury. However, the underlying mechanisms remain unclear. In the present study, it was revealed that sepsis or lipopolysaccharide (LPS) enhanced aerobic glycolysis as evidenced by increased lactate production and upregulated mRNA expression of glycolysis-related genes in kidney tissues and human renal tubular epithelial (HK-2) cells. The aerobic glycolysis inhibitor 2-deoxy-D-glucose (2-DG) downregulated glycolysis, and improved kidney injury induced by sepsis. 2-DG treatments increased the expression of sirtuin 3 (SIRT3) and phosphorylation-AMP-activated protein kinase (p-AMPK), following promoted autophagy and attenuated apoptosis of tubular epithelial cells in septic mice and in LPS-treated HK-2 cells. However, the glycolysis metabolite lactate downregulated SIRT3 and p-AMPK expression, inhibited autophagy and enhanced apoptosis in LPS-treated HK-2 cells. Furthermore, pharmacological blockade of autophagy with 3-methyladenine (3-MA) partially abolished the protective effect of 2-DG in sepsis-induced AKI. These findings indicated that inhibition of aerobic glycolysis protected against sepsis-induced AKI by promoting autophagy via the lactate/SIRT3/AMPK pathway.
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