期刊
DIABETES RESEARCH AND CLINICAL PRACTICE
卷 119, 期 -, 页码 106-117出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.diabres.2016.07.003
关键词
Gestational diabetes mellitus; Sex hormone-binding globulin; Insulin receptor substrate; Phosphatidylinositol 3-kinase; Glucose transport protein
资金
- NSFC (Natural Science Foundation of China) [81170591, 81300511]
- science and technology project of Shenyang [F11-262-9-46]
Objective: The aim of the present study was to investigate the probable pathogenesis of gestational diabetes mellitus (GDM) by analyzing the correlation between sex hormone-binding globulin (SHBG) secreted by the placenta during pregnancy and insulin signaling components and glucose transporter proteins (GLUTs) in the placental tissue. Design and methods: Placental tissue was collected from full-term and non-obese [body mass index <25 kg/m(2)] pregnant women; 10 diagnosed with GDM and 10 with normal pregnancy. We used real-time polymerase chain reaction (PCR), immunohistochemistry and western blotting to detect expression of protein and mRNA of SHBG and insulin signaling components and GLUTs in placental tissue. Results: In the placental tissue of non-obese women, there was a decrease in expression of SHBG protein and mRNA, with a concurrent decrease in expression of GLUT-4 protein and mRNA in women with GDM compared with normal controls. There was a decrease in GLUT-3 and insulin receptor substrate (IRS)-1 protein expression and lower IRS-2 mRNA expression was also observed in GDM placental tissue. Linear correlation analyses showed a positive correlation between SHBG and IRS-2 mRNA (P = 0.038, R-2 = 0.2178, y = 0.249x + 1.4208); positive correlation between SHBG and phosphatidylinositol 3-kinase (PI3K) p85 alpha mRNA (P = 0.035, R-2 = 0.224, y = 0.3506x + 0.7433); positive correlation between SHBG and GLUT-4 mRNA (P = 0.000, R-2 = 0.5174, y = 1.3822 + 1.7811x); positive correlation between IRS-2 and GLUT-4 mRNA (P = 0.002, R-2 = 0.4064, y = -0.8272 + 2.9592x); negative correlation between IRS-1 and PI3K p85 alpha mRNA (P = 0.005, R-2 = 0.366, y = 2.4492-0.1929x); negative correlation between IRS-1 and GLUT-3 mRNA (P = 0.027, R-2 = 0.243, y = 0.9254-0.0714x); and positive correlation between IRS-2 and GLUT-1 mRNA (P = 0.004, R-2 = 0.3794, y = 0.0225 + 0.6298x). Conclusion: The results confirm that defective receptors for insulin signal transduction and GLUT proteins are present in GDM placental tissue. Decreasing expression of SHBG may participate in regulation of insulin signaling, leading to a concomitant decrease in expression of relevant insulin signaling components in placental tissue, implying insulin resistance and eventual development of GDM. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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