期刊
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
卷 46, 期 5, 页码 3716-3725出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2020.10.246
关键词
PICRUSt; Microbial community; Biohydrogen; Fermentation
资金
- National Natural Science Foundation of China [51338005]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT-13026]
This study used PICRUSt to analyze the changes in microbial community functions in a biohydrogen production system, revealing that ionizing radiation pretreatment altered metabolic flux and decreased homoacetogenic gene, leading to increased hydrogen production, but also resulted in reduced carbohydrate metabolism.
PICRUSt (phylogenetic investigation of communities by reconstruction of unobserved states) is based on 16S rRNA sequencing data, which can analyze the microbial functions in fermentation system. In this study, PICRUSt was adopted to figure out the direct evidence of functions of the microbial community in biohydrogen production system. PICRUSt analysis demonstrated that metabolic flux shifted from acid-producing pathway to hydrogen-producing pathway, and the hydrogen-consuming homoacetogenic pathway was eliminated by ionizing radiation pretreatment at 5 kGy. KEGG (Kyoto Encyclopedia of Genes and Genomes) based functional genes analysis showed enriched energy metabolism and diminished homoaceto gene, which enhanced the hydrogen production. However, the diminished carbohydrate metabolism indicated that the pretreatment reduced the activity of microbial consortia to degrade various substrates. This study suggested that PICRUSt is an effective approach to analyze the functional profiling of microbial community in fermentative hydrogen production system. (C) 2020 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.
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