期刊
INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
卷 26, 期 3, 页码 532-542出版社
SPRINGER JAPAN KK
DOI: 10.1007/s10147-020-01832-5
关键词
TRIM37; Sorafenib-resistance; Hepatocellular carcinoma; AKT signaling
类别
资金
- Natural Science Foundation of China [81602701, 81974443]
- Natural Science Foundation of Guangdong Province [2017A030313547, 2018A030313176]
- Science and Technology Projects Foundation of Guangdong Province [2015A070710006, 2016A020215053]
- Science and Technology Projects Foundation of Guangzhou City [201507020037, 201607010260]
- Research project of Gannan Medical University, Gannan Medical University Key Cultivation project [ZD201802]
- Natural Science Foundation of Jiangxi Province [20202BAB206040]
The study showed that TRIM37 expression was significantly increased in HCC cells and tissues, especially in Sorafenib-resistant HCC tissues. High TRIM37 expression was correlated with poor prognosis in HCC patients. TRIM37 overexpression conferred Sorafenib resistance on HCC cells, while inhibition of TRIM37 sensitized HCC cells to Sorafenib cytotoxicity. Additionally, TRIM37 upregulated AKT activity and phosphorylated AKT levels, thereby activating canonical AKT signaling.
Background Hepatocellular carcinoma (HCC) is the most common primary liver cancer in the worldwide. Sorafenib is approved for first-line therapy against advanced HCC, but chemo-resistance is still a leading cause of tumor relapse and treatment failure in HCC. Thus, there is a significant clinical need to identify effective strategies to overcome drug resistance on the disease. Methods The protein and mRNA expression of TRIM37 in HCC cell lines and patient tissues were determined using Real-time PCR and Western blot, respectively. HCC tissue samples were analyzed by IHC to investigate the association between TRIM37expression and the clinicopathological characteristics of HCC patients. Functional assays, such as MTT, FACS, and Tunel assay, are used to determine the oncogenic role of TRIM37 in human HCC progression. Furthermore, western blotting and luciferase assay were used to determine the mechanism of TRIM37promotes chemoresistance in HCC. Results We found that both the mRNA and protein expression of TRIM37 was markedly upregulated in HCC cell lines and tissues, especially in Sorafenib-resistance HCC tissues. Moreover, high TRIM37 expression was associated with poor prognosis with HCC patients. TRIM37 overexpression confers Sorafenib resistance on HCC cells; however, inhibition of TRIM37 sensitized HCC cell lines to Sorafenib cytotoxicity. Additionally, TRIM37 upregulated the levels of AKT activity and phosphorylated AKT, thereby activating canonical AKT signaling. Conclusion Our findings suggest that targeting TRIM37 signaling may represent a promising strategy to enhance Sorafenib response in HCC patients with chemoresistant.
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