期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 168, 期 -, 页码 743-749出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.11.131
关键词
Pleurotus djamor; Galactoglucan; Antioxidant activity
资金
- DST, Govt. of India [SR/FST/College-295]
A new water soluble galactoglucan was isolated from the edible mushroom Pleurotus djamor, showing potential as a natural antioxidant with high antioxidant activity.
A new water soluble galactoglucan with apparent molecular weight similar to 1.61 x 10(5) Da, was isolated from the edible mushroom Pleurotus djamor by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography. The sugar analysis showed the presence of glucose and galactose in a molar ratio of nearly 3:1 respectively. The structure of the repeating unit in the polysaccharide was determined through chemical and NMR experiments as:-> 6)-beta-D-Glcp-(1 -> 4)-beta-D-Glcp-(1 -> 6)-beta-D-Glcp-(1 -> 3 up arrow 1-beta-D-Glcp In vitro antioxidant studies showed that the PDPS exhibited hydroxyl radical scavenging activity (EC50 = 1.681 +/- 0.034 mg/mL), DPPH radical scavenging activity (EC50 = 3.83 +/- 0.427 mg/mL), reducing power (EC50 = 4.258 +/- 0.095 mg/mL), and ABTS radical quenching activity (EC50 = 0.816 +/- 0.077 mg/mL). So, PDPS should be explored as a natural antioxidant. (C) 2020 Elsevier B.V. All rights reserved.
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