Hierarchical porous silk fibroin/poly(L-lactic acid) fibrous membranes towards vascular scaffolds

Fibrous membranes played an important role to prepare tubular scaffolds for muscular artery regeneration. In this study, a strategy has been developed to combine silk fibroin (SF) with highly porous electrospun poly(L-lactic acid) (PLLA) fibrous membrane towards vascular scaffolds. After PLLA fibres were electrospun and collected, they were immersed into acetone to generate a porous structure with ultra-high surface area. While the pores on PLEA fibres were fulfilled with SF solution and dried, SF was coated uniformly and tightly on PLEA fibres. A multilayer tubular structure of the tunica media was simulated by winding and stacking a strip of electrospun fibrous membrane. In vitro viability and morphology studies of A7r5 smooth muscle cells were undertaken for up to 14 days. Because the hydrophil icily of SF/PLLA composite fibres were improved dramatically, it had a positive effect on cell adhesion rate (97%) and proliferation (64.4%). Moreover, good cell morphology was observed via a multiphoton laser confocal microscope on SF/PLLA bioactive materials. These results demonstrated that the hierarchical porous SF/PLEA fibrous membranes are promising off-the-shelf scaffolds for muscular artery regeneration.

Automated summary

This study demonstrated that the fibrous membranes play a crucial role in preparing tubular scaffolds for muscular artery regeneration. By combining SF with PLLA, the scaffold showed significantly improved cell adhesion rate and proliferation, indicating promising potential for future applications.