期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 166, 期 -, 页码 550-556出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.10.212
关键词
Sar1; Procambarus clarkii; Immune response; Ecdysteroid signaling
资金
- Anhui Provincial Natural Science Foundation [1908085MC77]
- Natural Science Foundations of Anhui Higher Education Institutions of China [KJ2017A145]
Sar1 gene Pc-sar1 is expressed in Procambarus clarkii with varying levels in different tissues, and its RNA interference affects the expression of immune and hormone-related genes. It plays a role in innate immune response and ecdysteroid signaling pathway.
Secretion-associated and ras-related protein 1 (Sar1) is a small GTPase that plays an important role in the transport of protein coated with coat protein complex II vesicles. However, its alternative roles in the biological processes of Procambarus clarkii remain unclear. Here, a sar1 gene (named as Pc-sar1) with an open reading frame of 582 bp from P. clarkii was identified. Pc-sar1 was expressed in all examined tissues with highest expression levels in muscle, which was determined by real-time PCR and western blotting. After the induction of lipopolysaccharide (LPS) and polycytidylic acid (Poly I: C), the transcriptional levels of Pc-sar1 differed in hepatopancreas, gill, musde and intestine. In contrast, the expression of Pc-sar1 was upregulated by 20-hydroxyecdysone in these four tissues. In addition, the RNA interference of Pc-sar1 significantly affected the expression levels of immune and hormone-related genes. These results indicate that Pc-sar1 is involved in the innate immune response and ecdysteroid signaling pathway. (C) 2018 Elsevier B.V. All rights reserved.
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